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State Level Eligibility Test

SLET: Life Sciences

Code No. : 04
Subject : LIFE SCIENCES
PAPER III

33. Principles and methods of genetic engineering and Gene targeting; Applications in agriculture, health and industry.

34. Cell and tissue culture in plants and animals; Primary culture; Cell line; Cell clones; Callus
cultures; Somaclonal variation; Micropropagation; Somatic embryogenesis; Haploidy; Protoplast fusion and somatic hydridization; Cybrids; Gene transfer methods in plants and in animals; Transgenic biology; Allopheny; Aritificial seeds; Hybridoma technology.

35. Structure and organisation of membranes; Glycoconjugates and proteins in membrane systems; Ion transport, Na+/K+ATPase; Molecular basis of signal transduction in bacteria, plants and animals; Model membranes; Liposomes.

36. Principles and application of light, phase contrast, fluorescence, scanning and transmission
electron microscopy; Cytophotometry and flow cytometry, fixation and staining.

37. Principles and applications of gel-filtration, ion-exchange and affinity chromatography; Thin
layer and gas chromatography; High pressure liquid chromatography (HPLC); Electrophoresis
and electrofocussing; Ultracentrifugation (velocity and buoyant density).

38. Principles and techniques of nucleic acid hybridization and Cot curves; Sequencing of proteins and nucleic acids; Southern, Northern and South-Westem blotting techniques; Polymerase chain reaction; Methods for measuring nucleic acid and protein interactions.